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PEBP1 Monoclonal antibody

PEBP1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D4D11

Cat no : 66438-1-Ig

Print datasheet

Synonyms

RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3

验证数据展示

  • WB analysis of fetal human brain using 66438-1-Ig

    WB analysis of fetal human brain using 66438-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66438-1-Ig

    IHC staining of human prostate cancer using 66438-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IHC staining of human prostate cancer using 66438-1-Ig

    IHC staining of human prostate cancer using 66438-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IF Staining of PC-12 using 66438-1-Ig

    IF Staining of PC-12 using 66438-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of A549 using 66438-1-Ig

    IF Staining of A549 using 66438-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of PC-12 using 66438-1-Ig

    FC experiment of PC-12 using 66438-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human PEBP1 (66438-1-Ig, Clone:2D4D11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected infetal human brain tissue, Neuro-2a cells, PC-12 cells, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy PEBP1 Ready-To-Use IHC Kit. PEBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66438-1-Ig targets PEBP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PEBP1 fusion protein Ag0864 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylethanolamine binding protein 1
Synonyms RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC008714
Gene Symbol PEBP1
Gene ID (NCBI) 5037
RRIDAB_2881808
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

实验方案

Product Specific Protocols
WB protocol for PEBP1 antibody 66438-1-IgDownload protocol
IHC protocol for PEBP1 antibody 66438-1-IgDownload protocol
IF protocol for PEBP1 antibody 66438-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Heliyon

Metabolomics reveals that ferroptosis participates in bisphenol A-induced testicular injury

Authors - Ling Kan Chi
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