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验证数据展示

  • WB analysis of fetal human brain using 66438-1-Ig (same clone as 66438-1-PBS)

    WB analysis of fetal human brain using 66438-1-Ig (same clone as 66438-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IF Staining of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    IF Staining of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66438-1-Ig (same clone as 66438-1-PBS)

    IF Staining of A549 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    FC experiment of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human PEBP1 (66438-1-Ig, Clone:2D4D11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

PEBP1 Monoclonal antibody, PBS Only

PEBP1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66438-1-PBS

产品说明书

CloneNo. 2D4D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PEBP1 Ready-To-Use IHC Kit. PEBP1 primary antibody included.

产品信息

66438-1-PBS targets PEBP1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PEBP1 fusion protein Ag0864 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylethanolamine binding protein 1
别名 RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3
计算分子量 21 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC008714
基因名称 PEBP1
Gene ID (NCBI) 5037
RRIDAB_2881808
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30086
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.