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PEBP1 Monoclonal antibody, PBS Only

PEBP1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4D11

Cat no : 66438-1-PBS

Print datasheet

Synonyms

RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3

验证数据展示

  • WB analysis of fetal human brain using 66438-1-Ig (same clone as 66438-1-PBS)

    WB analysis of fetal human brain using 66438-1-Ig (same clone as 66438-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IF Staining of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    IF Staining of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66438-1-Ig (same clone as 66438-1-PBS)

    IF Staining of A549 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    FC experiment of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human PEBP1 (66438-1-Ig, Clone:2D4D11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PEBP1 Ready-To-Use IHC Kit. PEBP1 primary antibody included.

产品信息

66438-1-PBS targets PEBP1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PEBP1 fusion protein Ag0864 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylethanolamine binding protein 1
Synonyms RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC008714
Gene Symbol PEBP1
Gene ID (NCBI) 5037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30086
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.