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验证数据展示

  • FC experiment of PC-12 using CL488-66438

    FC experiment of PC-12 using CL488-66438

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PEBP1 (CL488-66438, Clone:2D4D11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using CL488-66438

    IF Staining of A549 using CL488-66438

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using CoraLite® Plus 488 PEBP1 antibody (CL488-66438, Clone: 2D4D11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PEBP1 Monoclonal antibody

PEBP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66438

产品说明书

CloneNo. 2D4D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy PEBP1 Ready-To-Use IHC Kit. PEBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66438-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66438 targets PEBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PEBP1 fusion protein Ag0864 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylethanolamine binding protein 1
别名 RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3
计算分子量 21 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC008714
基因名称 PEBP1
Gene ID (NCBI) 5037
RRIDAB_2919325
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30086
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PEBP1 antibody CL488-66438Download protocol
Standard Protocols
Click here to view our Standard Protocols