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Serpin F1/PEDF Monoclonal antibody

Serpin F1/PEDF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C5C8

Cat no : 66554-1-Ig

Print datasheet

Synonyms

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

验证数据展示

  • WB analysis of human plasma using 66554-1-Ig

    WB analysis of human plasma using 66554-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma tissue, pig plasma tissue, human blood tissue
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66554-1-Ig targets Serpin F1/PEDF in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, pig
Immunogen Serpin F1/PEDF fusion protein Ag26660 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Synonyms PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC013984
Gene Symbol PEDF
Gene ID (NCBI) 5176
RRIDAB_2881916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

实验方案

Product Specific Protocols
WB protocol for Serpin F1/PEDF antibody 66554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols