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验证数据展示

  • WB analysis of human plasma using 66554-1-Ig

    WB analysis of human plasma using 66554-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Serpin F1/PEDF Monoclonal antibody

Serpin F1/PEDF Monoclonal Antibody for WB, ELISA
Cat No. 66554-1-Ig

产品说明书

CloneNo. 2C5C8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, ELISA

别名

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, pig plasma tissue, human blood tissue
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66554-1-Ig targets Serpin F1/PEDF in WB, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig
免疫原 Serpin F1/PEDF fusion protein Ag26660 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
别名 PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein
计算分子量 418 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC013984
基因名称 PEDF
Gene ID (NCBI) 5176
RRIDAB_2881916
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

实验方案

Product Specific Protocols
WB protocol for Serpin F1/PEDF antibody 66554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols