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CoraLite® Plus 488-conjugated Serpin F1/PEDF Monoclonal antibody

Serpin F1/PEDF Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C5C8

Cat no : CL488-66554

Print datasheet

Synonyms

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

验证数据展示

  • WB analysis using CL488-66554

    WB analysis using CL488-66554

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66554 (PEDF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig plasma, human plasma
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66554-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66554 targets Serpin F1/PEDF in WB applications and shows reactivity with human, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, pig
Immunogen Serpin F1/PEDF fusion protein Ag26660 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Synonyms PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC013984
Gene Symbol PEDF
Gene ID (NCBI) 5176
RRIDAB_3084258
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Serpin F1/PEDF antibody CL488-66554Download protocol
Standard Protocols
Click here to view our Standard Protocols