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验证数据展示

  • WB analysis using CL488-66554

    WB analysis using CL488-66554

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66554 (PEDF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated Serpin F1/PEDF Monoclonal antibody

Serpin F1/PEDF Monoclonal Antibody for WB
Cat No. CL488-66554

产品说明书

CloneNo. 2C5C8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB

别名

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig plasma, human plasma
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66554-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66554 targets Serpin F1/PEDF in WB applications and shows reactivity with human, pig samples.

经测试应用 WB Application Description
经测试反应性 human, pig
免疫原 Serpin F1/PEDF fusion protein Ag26660 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
别名 PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein
计算分子量 418 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC013984
基因名称 PEDF
Gene ID (NCBI) 5176
RRIDAB_3084258
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP36955
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Serpin F1/PEDF antibody CL488-66554Download protocol
Standard Protocols
Click here to view our Standard Protocols