• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

    WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included
  • IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included
  • IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

    FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IF Staining of mouse breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

PELP1 Monoclonal antibody, PBS Only

PELP1 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA
Cat No. 67050-1-PBS

产品说明书

CloneNo. 1B11E4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

别名

1B11E4, HMX3, MNAR, Modulator of non-genomic activity of estrogen receptor, Proline-, glutamic acid- and leucine-rich protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PELP1 Ready-To-Use IHC Kit. PELP1 primary antibody included.

产品信息

67050-1-PBS targets PELP1 in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PELP1 fusion protein Ag25729 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proline, glutamate and leucine rich protein 1
别名 1B11E4, HMX3, MNAR, Modulator of non-genomic activity of estrogen receptor, Proline-, glutamic acid- and leucine-rich protein 1
计算分子量 1130 aa, 120 kDa
观测分子量 160 kDa
GenBank蛋白编号BC069058
基因名称 PELP1
Gene ID (NCBI) 27043
RRIDAB_2882363
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZL8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.