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PELP1 Monoclonal antibody, PBS Only

PELP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11E4

Cat no : 67050-1-PBS

Print datasheet

Synonyms

Transcription factor HMX3, Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, MNAR, HMX3

验证数据展示

  • WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

    WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included
  • IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included
  • IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

    FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PELP1 Ready-To-Use IHC Kit. PELP1 primary antibody included.

产品信息

67050-1-PBS targets PELP1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen PELP1 fusion protein Ag25729 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proline, glutamate and leucine rich protein 1
Synonyms Transcription factor HMX3, Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, MNAR, HMX3
Calculated Molecular Weight 1130 aa, 120 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC069058
Gene Symbol PELP1
Gene ID (NCBI) 27043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZL8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.