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Profilin 1 Monoclonal antibody
Profilin 1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
3A12E8
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HAP1, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HAP1 cells |
Planning an IHC experiment? We recommend our IHCeasy PFN1 Ready-To-Use IHC Kit. PFN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67390-1-Ig targets Profilin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Immunogen | Profilin 1 fusion protein Ag29206 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | profilin 1 |
| Synonyms | PFN1, profilin 1, Profilin I |
| Calculated Molecular Weight | 140 aa, 15 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC006768 |
| Gene Symbol | Profilin 1 |
| Gene ID (NCBI) | 5216 |
| RRID | AB_2882634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07737 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Profilin 1 antibody 67390-1-Ig | Download protocol |
| IHC protocol for Profilin 1 antibody 67390-1-Ig | Download protocol |
| IF protocol for Profilin 1 antibody 67390-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Prolif An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms | ||
iScience Methods to separate nuclear soluble fractions reflecting localizations in living cells | ||
Diabetes Metab Syndr Obes Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway | ||
F1000Res The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence
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