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验证数据展示

  • IF Staining of HeLa using CL488-67390

    IF Staining of HeLa using CL488-67390

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Profilin 1 antibody (CL488-67390, Clone: 3A12E8 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 488-conjugated Profilin 1 Monoclonal antibody

Profilin 1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67390

产品说明书

CloneNo. 3A12E8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

PFN1, profilin 1, Profilin I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PFN1 Ready-To-Use IHC Kit. PFN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67390-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67390 targets Profilin 1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原 Profilin 1 fusion protein Ag29206 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 profilin 1
别名 PFN1, profilin 1, Profilin I
计算分子量 140 aa, 15 kDa
观测分子量 13 kDa
GenBank蛋白编号BC006768
基因名称 Profilin 1
Gene ID (NCBI) 5216
RRIDAB_3084343
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07737
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Profilin 1 antibody CL488-67390Download protocol
Standard Protocols
Click here to view our Standard Protocols