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验证数据展示

  • IF Staining of HeLa using CL488-67390

    IF Staining of HeLa using CL488-67390

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Profilin 1 antibody (CL488-67390, Clone: 3A12E8 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Profilin 1 Monoclonal antibody

Profilin 1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67390

Print datasheet

CloneNo. 3A12E8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

PFN1, profilin 1, Profilin I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PFN1 Ready-To-Use IHC Kit. PFN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67390-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67390 targets Profilin 1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Profilin 1 fusion protein Ag29206 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name profilin 1
Synonyms PFN1, profilin 1, Profilin I
Calculated Molecular Weight 140 aa, 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC006768
Gene Symbol Profilin 1
Gene ID (NCBI) 5216
RRIDAB_3084343
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07737
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Profilin 1 antibody CL488-67390Download protocol
Standard Protocols
Click here to view our Standard Protocols