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  • Featured Product
  • KD/KO Validated

Profilin 1 Monoclonal antibody, PBS Only

Profilin 1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12E8

Cat no : 67390-1-PBS

Print datasheet

Synonyms

PFN1, profilin 1, Profilin I

验证数据展示

  • WB analysis using 67390-1-Ig (same clone as 67390-1-PBS)

    WB analysis using 67390-1-Ig (same clone as 67390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67390-1-Ig (same clone as 67390-1-PBS)

    IF Staining of HeLa using 67390-1-Ig (same clone as 67390-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Profilin 1 antibody (67390-1-Ig, Clone: 3A12E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

  • IF Staining of HAP1 using 67390-1-Ig (same clone as 67390-1-PBS)

    IF Staining of HAP1 using 67390-1-Ig (same clone as 67390-1-PBS)

    HAP1 WT cells (green outline) and PFN1 KO cells (magenta outline) labelled with a green or a far-red fluorescence dye, respectively. Cells with 4% PFA and stained with 67390-1-lg at 1.0 μg/ml plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67390-1-Ig (same clone as 67390-1-PBS)

    WB analysis of HeLa using 67390-1-Ig (same clone as 67390-1-PBS)

    WB result of Profilin 1 antibody (67390-1-Ig; 1:160000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Profilin 1 transfected HeLa cells. This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 67390-1-Ig (same clone as 67390-1-PBS)

    WB analysis of HAP1 using 67390-1-Ig (same clone as 67390-1-PBS)

    HAP1 (WT and PFN1 KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 67390-1-lg  incubated at 1:30,000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67390-1-Ig (same clone as 67390-1-PBS)

    IHC staining of human colon cancer using 67390-1-Ig (same clone as 67390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67390-1-Ig (Profilin 1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67390-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFN1 antibody included
Planning an IHC experiment? We recommend our IHCeasy PFN1 Ready-To-Use IHC Kit. PFN1 primary antibody included.

产品信息

67390-1-PBS targets Profilin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Profilin 1 fusion protein Ag29206 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name profilin 1
Synonyms PFN1, profilin 1, Profilin I
Calculated Molecular Weight 140 aa, 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC006768
Gene Symbol Profilin 1
Gene ID (NCBI) 5216
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07737
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.