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CoraLite® Plus 488-conjugated PGAM5 Polyclonal antibody

PGAM5 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-28445

Print datasheet

Synonyms

BXLBv68, PGAM5

验证数据展示

  • IF Staining of MCF-7 using CL488-28445

    IF Staining of MCF-7 using CL488-28445

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PGAM5 antibody (CL488-28445) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 28445-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28445 targets PGAM5 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen PGAM5 fusion protein Ag28195 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoglycerate mutase family member 5
Synonyms BXLBv68, PGAM5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC008196
Gene Symbol PGAM5
Gene ID (NCBI) 192111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HS1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
FC protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
Standard Protocols
Click here to view our Standard Protocols