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验证数据展示

  • IF Staining of MCF-7 using CL488-28445

    IF Staining of MCF-7 using CL488-28445

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PGAM5 antibody (CL488-28445) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of MCF-7 using CL488-28445

    FC experiment of MCF-7 using CL488-28445

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pgam5 Polyclonal Antibody (CL488-28445)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PGAM5 Polyclonal antibody

PGAM5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28445

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Serine/threonine-protein phosphatase PGAM5, mitochondrial, EC:3.1.3.16, BXLBv68, Bcl-XL-binding protein v68

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 28445-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28445 targets PGAM5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PGAM5 fusion protein Ag28195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglycerate mutase family member 5
别名 Serine/threonine-protein phosphatase PGAM5, mitochondrial, EC:3.1.3.16, BXLBv68, Bcl-XL-binding protein v68
计算分子量 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC008196
基因名称 PGAM5
Gene ID (NCBI) 192111
RRIDAB_3672826
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HS1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
FC protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
Standard Protocols
Click here to view our Standard Protocols