CoraLite® Plus 488-conjugated PHD2/EGLN1 Monoclonal antibody
PHD2/EGLN1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat , Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A2F1
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy PHD2/EGLN1 Ready-To-Use IHC Kit. PHD2/EGLN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66589-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66589 targets PHD2/EGLN1 in IF/ICC applications and shows reactivity with Human, Mouse , Rat , Pig samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse , Rat , Pig |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | egl nine homolog 1 (C. elegans) |
Synonyms | C1orf12, DKFZp761F179, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PHD2/EGLN1, PNAS 137, SM 20, SM20, ZMYND6 |
Calculated Molecular Weight | 46 kDa |
GenBank Accession Number | NM_022051 |
Gene Symbol | PHD2/EGLN1 |
Gene ID (NCBI) | 54583 |
RRID | AB_2919349 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9GZT9 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 PHD2/EGLN1 antibody CL488-66589 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |