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验证数据展示

  • IF Staining of HEK-293 using CL488-66589

    IF Staining of HEK-293 using CL488-66589

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 PHD2/EGLN1 antibody (CL488-66589, Clone: 1A2F1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66589

产品说明书

CloneNo. 1A2F1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse , Rat , Pig

应用

IF/ICC

别名

C1orf12, DKFZp761F179, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PHD2/EGLN1, PNAS 137, SM 20, SM20, ZMYND6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PHD2/EGLN1 Ready-To-Use IHC Kit. PHD2/EGLN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66589-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66589 targets PHD2/EGLN1 in IF/ICC applications and shows reactivity with Human, Mouse , Rat , Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse , Rat , Pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 egl nine homolog 1 (C. elegans)
别名 C1orf12, DKFZp761F179, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PHD2/EGLN1, PNAS 137, SM 20, SM20, ZMYND6
计算分子量 46 kDa
GenBank蛋白编号NM_022051
基因名称 PHD2/EGLN1
Gene ID (NCBI) 54583
RRIDAB_2919349
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZT9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PHD2/EGLN1 antibody CL488-66589Download protocol
Standard Protocols
Click here to view our Standard Protocols