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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A2F1

Cat no : CL594-66589

Print datasheet

Synonyms

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

验证数据展示

  • IF Staining of HEK-293 using CL594-66589

    IF Staining of HEK-293 using CL594-66589

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®®594 PHD2/EGLN1 antibody (CL594-66589, Clone: 1A2F1 ) at dilution of 1:200.

  • IF Staining of HEK-293 using CL594-66589

    IF Staining of HEK-293 using CL594-66589

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 PHD2/EGLN1 antibody (CL594-66589, Clone: 1A2F1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PHD2/EGLN1 Ready-To-Use IHC Kit. PHD2/EGLN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66589-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66589 targets PHD2/EGLN1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name egl nine homolog 1 (C. elegans)
Synonyms EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29
Calculated Molecular Weight 46 kDa
GenBank Accession NumberNM_022051
Gene Symbol PHD2/EGLN1
Gene ID (NCBI) 54583
RRIDAB_2920011
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZT9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

实验方案

Product Specific Protocols
IF protocol for CL594 PHD2/EGLN1 antibody CL594-66589Download protocol
Standard Protocols
Click here to view our Standard Protocols