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验证数据展示

  • IF Staining of SH-SY5Y using CL488-25276

    IF Staining of SH-SY5Y using CL488-25276

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 PHOX2B antibody (CL488-25276) at dilution of 1:600, CL594-phalloidin (red).

  • FC experiment of Neuro-2a using CL488-25276

    FC experiment of Neuro-2a using CL488-25276

    1x10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PHOX2B Polyclonal antibody (CL488-25276)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated PHOX2B Polyclonal antibody

PHOX2B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25276

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

PMX2B, Paired-like homeobox 2B, paired like homeobox 2b, Neuroblastoma Phox, NBPhox

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inNeuro-2a cells
For other applications, we recommend the unconjugated version of this antibody, 25276-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25276 targets PHOX2B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 PHOX2B fusion protein Ag13196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paired-like homeobox 2b
别名 PMX2B, Paired-like homeobox 2B, paired like homeobox 2b, Neuroblastoma Phox, NBPhox
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC017199
基因名称 PHOX2B
Gene ID (NCBI) 8929
RRIDAB_3672790
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99453
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PHOX2B antibody CL488-25276Download protocol
FC protocol for CL Plus 488 PHOX2B antibody CL488-25276Download protocol
Standard Protocols
Click here to view our Standard Protocols