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验证数据展示

  • FC experiment of HeLa using CL555-67121

    FC experiment of HeLa using CL555-67121

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PI3 Kinase p110 Beta (CL555-67121, Clone:1B2A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®555-conjugated PI3 Kinase p110 Beta Monoclonal antibody

PI3 Kinase p110 Beta Monoclonal Antibody for FC (Intra)
Cat No. CL555-67121

产品说明书

CloneNo. 1B2A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat

应用

FC (Intra)

别名

DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67121-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67121 targets PI3 Kinase p110 Beta in FC (Intra) applications and shows reactivity with Human, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Rat
免疫原 PI3 Kinase p110 Beta fusion protein Ag17505 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, beta polypeptide
别名 DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta
计算分子量 1070 aa, 123 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC114432
基因名称 PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_3084628
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP42338
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

实验方案

Product Specific Protocols
FC protocol for CL555 PI3 Kinase p110 Beta antibody CL555-67121Download protocol
Standard Protocols
Click here to view our Standard Protocols