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验证数据展示

  • IF Staining of HeLa using CL594-67121

    IF Staining of HeLa using CL594-67121

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PI3 Kinase p110 Beta antibody (CL594-67121, Clone: 1B2A1 ) at dilution of 1:200. DAPI (blue).

CoraLite®594-conjugated PI3 Kinase p110 Beta Monoclonal antibody

PI3 Kinase p110 Beta Monoclonal Antibody for IF/ICC
Cat No. CL594-67121

产品说明书

CloneNo. 1B2A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat

应用

IF/ICC

别名

DKFZp779K1237, p110 BETA, PI3 kinase subunit beta, PI3K, PI3K beta, PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67121-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67121 targets PI3 Kinase p110 Beta in IF/ICC applications and shows reactivity with Human, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Rat
免疫原 PI3 Kinase p110 Beta fusion protein Ag17505 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, beta polypeptide
别名 DKFZp779K1237, p110 BETA, PI3 kinase subunit beta, PI3K, PI3K beta, PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta
计算分子量 1070 aa, 123 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC114432
基因名称 PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2920089
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP42338
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

实验方案

Product Specific Protocols
IF protocol for CL594 PI3 Kinase p110 Beta antibody CL594-67121Download protocol
Standard Protocols
Click here to view our Standard Protocols