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PIAS1 Recombinant antibody, PBS Only

PIAS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C22

Cat no : 82873-2-PBS

Print datasheet

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

验证数据展示

  • WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82873-2-PBS

    Affinity and Kinetic Characterization of 82873-2-PBS

    Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

产品信息

82873-2-PBS targets PIAS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PIAS1 fusion protein Ag18132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein inhibitor of activated STAT, 1
Synonyms EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C