PIAS1 Recombinant antibody

PIAS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82873-2-RR
CloneNo. 1C22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82873-2-RR targets PIAS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIAS1 fusion protein Ag18132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein inhibitor of activated STAT, 1
Synonyms EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
RRIDAB_3670593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIAS1 antibody 82873-2-RRDownload protocol
IHC protocol for PIAS1 antibody 82873-2-RRDownload protocol
IF protocol for PIAS1 antibody 82873-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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