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验证数据展示

  • WB analysis using 82873-2-RR

    WB analysis using 82873-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 82873-2-RR

    IHC staining of mouse testis using 82873-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IHC staining of mouse testis using 82873-2-RR

    IHC staining of mouse testis using 82873-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IF Staining of HepG2 using 82873-2-RR

    IF Staining of HepG2 using 82873-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 82873-2-RR

    FC experiment of HeLa using 82873-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PIAS1 Recombinant antibody (82873-2-RR, Clone:1C22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82873-2-RR

    Affinity and Kinetic Characterization of 82873-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

PIAS1 Recombinant antibody

PIAS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82873-2-RR
CloneNo. 1C22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82873-2-RR targets PIAS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIAS1 fusion protein Ag18132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein inhibitor of activated STAT, 1
Synonyms EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
RRIDAB_3670593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIAS1 antibody 82873-2-RRDownload protocol
IHC protocol for PIAS1 antibody 82873-2-RRDownload protocol
IF protocol for PIAS1 antibody 82873-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols