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  • Featured Product
  • KD/KO Validated

PKM2-specific Monoclonal antibody, PBS Only

PKM2-specific Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11C7

Cat No : 60268-1-PBS

Print datasheet

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

验证数据展示

  • WB analysis of HEK-293 using 60268-1-Ig (same clone as 60268-1-PBS)

    WB analysis of HEK-293 using 60268-1-Ig (same clone as 60268-1-PBS)

    WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

  • WB analysis using 60268-1-Ig (same clone as 60268-1-PBS)

    WB analysis using 60268-1-Ig (same clone as 60268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

    IF Staining of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

    IP experiment of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

    IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

    IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

    IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PKM2 antibody included
Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.

产品信息

60268-1-PBS targets PKM2-specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PKM pyruvate kinase, muscle
Synonyms CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_002654
Gene Symbol PKM
Gene ID (NCBI) 5315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14618
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.