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验证数据展示

  • WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

    IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

    IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PLCD4 Polyclonal antibody, PBS Only

PLCD4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30133-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

30133-1-PBS targets PLCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PLCD4 fusion protein Ag32651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, delta 4
别名
计算分子量 85-90 kDa
观测分子量90 kDa
GenBank蛋白编号BC006355
基因名称 PLCD4
Gene ID (NCBI) 84812
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRC7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).