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  • Featured Product
  • KD/KO Validated

PLCD4 Polyclonal antibody, PBS Only

PLCD4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30133-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

    IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

    IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

产品信息

30133-1-PBS targets PLCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PLCD4 fusion protein Ag32651 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, delta 4
Synonyms
Calculated Molecular Weight 85-90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRC7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).