• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67471-1-Ig

    WB analysis using 67471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67471-1-Ig (PLCL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67471-1-Ig

    IF Staining of HeLa using 67471-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67471-1-Ig (PLCL2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67471-1-Ig

产品说明书

CloneNo. 1C8A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

Phospholipase C-L2, Phospholipase C-epsilon-2, Phospholipase C L2, KIAA1092, Inactive phospholipase C-like protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67471-1-Ig targets PLCL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PLCL2 fusion protein Ag11461 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase C-like 2
别名 Phospholipase C-L2, Phospholipase C-epsilon-2, Phospholipase C L2, KIAA1092, Inactive phospholipase C-like protein 2
计算分子量 126 kDa
观测分子量 126 kDa
GenBank蛋白编号BC036392
基因名称 PLCL2
Gene ID (NCBI) 23228
RRIDAB_2882700
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

实验方案

Product Specific Protocols
WB protocol for PLCL2 antibody 67471-1-IgDownload protocol
IF protocol for PLCL2 antibody 67471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols