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验证数据展示

  • IF Staining of HepG2 using CL594-67471

    IF Staining of HepG2 using CL594-67471

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 PLCL2 antibody (CL594-67471, Clone: 1C8A4 ) at dilution of 1:200.

CoraLite®594-conjugated PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for IF/ICC
Cat No. CL594-67471

产品说明书

CloneNo. 1C8A4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse, Pig

应用

IF/ICC

别名

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67471-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67471 targets PLCL2 in IF/ICC applications and shows reactivity with Human, Rat, Mouse, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Rat, Mouse, Pig
免疫原 PLCL2 fusion protein Ag11461 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase C-like 2
别名 FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2
计算分子量 126 kDa
观测分子量 126 kDa
GenBank蛋白编号BC036392
基因名称 PLCL2
Gene ID (NCBI) 23228
RRIDAB_2920142
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPR0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

实验方案

Product Specific Protocols
IF protocol for CL594 PLCL2 antibody CL594-67471Download protocol
Standard Protocols
Click here to view our Standard Protocols