验证数据展示

WB analysis of A431 using 21214-1-AP
WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.
WB analysis of THLE-2 using 21214-1-AP
WB result of PLOD2-Specific antibody (21214-1-AP 1:3000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells.
WB analysis using 21214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 21214-1-AP
DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 21214-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP experiment of mouse testis using 21214-1-AP
IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.
IHC staining of human liver cancer using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PLOD2 antibody included
IHC staining of human liver cancer using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PLOD2 antibody included
FC experiment of HeLa using 21214-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21214-1-AP

产品说明书

引用文献(50)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 647 PE

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (50)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Caco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human liver cancer tissue, human pancreas tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 36 publications below
IHC	See 21 publications below
IF	See 7 publications below

产品信息

21214-1-AP targets PLOD2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rabbit, zebrafish
免疫原	PLOD2-Specific fusion protein Ag15614 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
别名	PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4
计算分子量	758 aa, 85 kDa
观测分子量	87 kDa
GenBank蛋白编号	BC037169
基因名称	PLOD2
Gene ID (NCBI)	5352
RRID	AB_10733347
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O00469
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

实验方案

Product Specific Protocols
WB protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IHC protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IF protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IP protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB, IHC	J Clin Invest Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma. Authors - Yulong Chen View Article
zebrafish	WB	Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis Authors - Ying Liu View Article
human, mouse	WB	Cancer Discov Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis. Authors - Eisinger-Mathason T S Karin TS View Article
mouse	WB	Arthritis Rheumatol Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis. Authors - Akio Yamamoto View Article
human	WB	Proc Natl Acad Sci U S A Intratumoral oxygen gradients mediate sarcoma cell invasion. Authors - Daniel M Lewis View Article
mouse	IHC	Arthritis Rheum Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis. Authors - Remst D F G DF View Article

验证数据展示

WB analysis of A431 using 21214-1-AP

WB analysis of THLE-2 using 21214-1-AP

WB analysis using 21214-1-AP

WB analysis using 21214-1-AP

IF Staining of HeLa using 21214-1-AP

IP experiment of mouse testis using 21214-1-AP

IHC staining of human liver cancer using 21214-1-AP

IHC staining of human liver cancer using 21214-1-AP

FC experiment of HeLa using 21214-1-AP

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis

Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.

Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.