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验证数据展示

  • FC experiment of HeLa using CL647-21214

    FC experiment of HeLa using CL647-21214

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PLOD2-Specific (CL647-21214) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for FC (Intra)
Cat No. CL647-21214

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

LH2, Lysyl hydroxylase 2, PLOD2, PLOD2-Specific, TLH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21214-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-21214 targets PLOD2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PLOD2-Specific fusion protein Ag15614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
别名 LH2, Lysyl hydroxylase 2, PLOD2, PLOD2-Specific, TLH
计算分子量 758 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC037169
基因名称 PLOD2
Gene ID (NCBI) 5352
RRIDAB_2934945
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00469
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 PLOD2-Specific antibody CL647-21214Download protocol
Standard Protocols
Click here to view our Standard Protocols