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  • Featured Product
  • KD/KO Validated

PE-conjugated PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

Cat No : PE-21214

Print datasheet

Synonyms

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4

验证数据展示

  • FC experiment of HeLa using PE-21214

    FC experiment of HeLa using PE-21214

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PE-conjugated PLOD2-Specific Polyclonal antibody (PE-21214)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-21214 targets PLOD2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PLOD2-Specific fusion protein Ag15614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00469
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

PLOD2, also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

实验方案

Product Specific Protocols
FC protocol for PE PLOD2-Specific antibody PE-21214Download protocol
Standard Protocols
Click here to view our Standard Protocols