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验证数据展示

  • WB analysis of MKN-45 using 83449-4-RR

    WB analysis of MKN-45 using 83449-4-RR

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 83449-4-RR (PLXDC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83449-4-RR

    Affinity and Kinetic Characterization of 83449-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83449-4-RR against Human PLXDC2 were performed. The affinity constant is 42.5 pM.

PLXDC2 Recombinant antibody

PLXDC2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83449-4-RR

产品说明书

CloneNo. 240174A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83449-4-RR targets PLXDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PLXDC2 fusion protein Ag2940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 plexin domain containing 2
别名 UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2
计算分子量 480 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC012885
基因名称 PLXDC2
Gene ID (NCBI) 84898
RRIDAB_3671087
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6UX71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

实验方案

Product Specific Protocols
WB protocol for PLXDC2 antibody 83449-4-RRDownload protocol
IHC protocol for PLXDC2 antibody 83449-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols