验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy PNPT1 Ready-To-Use IHC Kit. PNPT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68309-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68309 targets PNPT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PNPT1 fusion protein Ag6290 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | polyribonucleotide nucleotidyltransferase 1 |
| 别名 | |
| 计算分子量 | 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC053660 |
| 基因名称 | PNPT1 |
| Gene ID (NCBI) | 87178 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8TCS8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PNPT1 antibody CL488-68309 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |