POMC Monoclonal antibody
POMC Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (2)
Applications
IHC, IF/ICC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3C12C7
验证数据展示
经过测试的应用
Positive IHC detected in | human pituitary tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
IF | See 11 publications below |
产品信息
66358-1-Ig targets POMC in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | POMC fusion protein Ag17311 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | proopiomelanocortin |
Synonyms | Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha, ACTH |
Calculated Molecular Weight | 267 aa, 29 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC065832 |
Gene Symbol | POMC |
Gene ID (NCBI) | 5443 |
RRID | AB_2881737 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P01189 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for POMC antibody 66358-1-Ig | Download protocol |
IF protocol for POMC antibody 66358-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma. | ||
Biomed Pharmacother 919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities. | ||
Reprod Sci FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age. | ||
Food Addit Contam Part A Chem Anal FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs. |