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验证数据展示

  • IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

  • IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

  • IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

    IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

    FC experiment of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human POMC (66358-1-Ig, Clone:3C12C7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

POMC Monoclonal antibody, PBS Only

POMC Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66358-1-PBS

产品说明书

CloneNo. 3C12C7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha, ACTH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66358-1-PBS targets POMC in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 POMC fusion protein Ag17311 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proopiomelanocortin
别名 Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha, ACTH
计算分子量 267 aa, 29 kDa
观测分子量 35 kDa
GenBank蛋白编号BC065832
基因名称 POMC
Gene ID (NCBI) 5443
RRIDAB_2881737
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01189
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.