• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

POP1 Polyclonal antibody, PBS Only

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12029-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

    WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

    IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

    IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

产品信息

12029-1-PBS targets POP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen POP1 fusion protein Ag2660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
Synonyms
Calculated Molecular Weight 1024 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC011529
Gene Symbol POP1
Gene ID (NCBI) 10940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99575
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.