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验证数据展示

  • IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83732-2-RR (same clone as 83732-2-PBS)

    FC experiment of U2OS using 83732-2-RR (same clone as 83732-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83732-2-RR (same clone as 83732-2-PBS)

    FC experiment of SH-SY5Y using 83732-2-RR (same clone as 83732-2-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00711-1

    Cytometric bead array standard curve of MP00711-1

    Cytometric bead array standard curve of MP00711-1, POT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83732-4-PBS. Detection antibody: 83732-2-PBS. Standard: Ag0875. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83732-2-RR

    Affinity and Kinetic Characterization of 83732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

POT1 Recombinant antibody, PBS Only (Detector)

POT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83732-2-PBS

产品说明书

CloneNo. 240688E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

POT1-like telomere end-binding protein, hPot1, 240688E5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83732-2-PBS targets POT1 as part of a matched antibody pair:

MP00711-1: 83732-4-PBS capture and 83732-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 POT1 fusion protein Ag0875 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POT1 protection of telomeres 1 homolog (S. pombe)
别名 POT1-like telomere end-binding protein, hPot1, 240688E5
计算分子量 71 kDa
GenBank蛋白编号BC002923
基因名称 POT1
Gene ID (NCBI) 25913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUX5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)