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验证数据展示

  • IF Staining of HeLa using 83732-2-RR

    IF Staining of HeLa using 83732-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83732-2-RR

    FC experiment of U2OS using 83732-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of SH-SY5Y using 83732-2-RR

    FC experiment of SH-SY5Y using 83732-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using 83732-2-RR

    IF Staining of HeLa using 83732-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83732-2-RR

    Affinity and Kinetic Characterization of 83732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

POT1 Recombinant antibody

POT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83732-2-RR

产品说明书

CloneNo. 240688E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

POT1-like telomere end-binding protein, hPot1, 240688E5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83732-2-RR targets POT1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 POT1 fusion protein Ag0875 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POT1 protection of telomeres 1 homolog (S. pombe)
别名 POT1-like telomere end-binding protein, hPot1, 240688E5
计算分子量 71 kDa
GenBank蛋白编号BC002923
基因名称 POT1
Gene ID (NCBI) 25913
RRIDAB_3671332
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)

实验方案

Product Specific Protocols
IF protocol for POT1 antibody 83732-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols