验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse lung tissue, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | mouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy PPM1G Ready-To-Use IHC Kit. PPM1G primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
15532-1-AP targets PPM1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | PPM1G fusion protein Ag7751 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform |
| Synonyms | PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC000057 |
| Gene Symbol | PPM1G |
| Gene ID (NCBI) | 5496 |
| RRID | AB_2169934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1G antibody 15532-1-AP | Download protocol |
| IHC protocol for PPM1G antibody 15532-1-AP | Download protocol |
| IF protocol for PPM1G antibody 15532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation. | ||
J Oncol Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development | ||
Cell Death Dis PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.
|
