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验证数据展示

  • IF Staining of MCF-7 using CL488-15532

    IF Staining of MCF-7 using CL488-15532

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PPM1G antibody (CL488-15532) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-15532

    FC experiment of MCF-7 using CL488-15532

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PPM1G (CL488-15532) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PPM1G Polyclonal antibody

PPM1G Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15532

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPM1G Ready-To-Use IHC Kit. PPM1G primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15532-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15532 targets PPM1G in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PPM1G fusion protein Ag7751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
别名 PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma
计算分子量 59 kDa
观测分子量 70 kDa
GenBank蛋白编号BC000057
基因名称 PPM1G
Gene ID (NCBI) 5496
RRIDAB_3083973
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15355
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PPM1G antibody CL488-15532Download protocol
Standard Protocols
Click here to view our Standard Protocols