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验证数据展示

  • IF Staining of MCF-7 using CL488-15532

    IF Staining of MCF-7 using CL488-15532

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PPM1G antibody (CL488-15532) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-15532

    FC experiment of MCF-7 using CL488-15532

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PPM1G (CL488-15532) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PPM1G Polyclonal antibody

PPM1G Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15532

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPM1G Ready-To-Use IHC Kit. PPM1G primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15532-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15532 targets PPM1G in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PPM1G fusion protein Ag7751 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Synonyms PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC000057
Gene Symbol PPM1G
Gene ID (NCBI) 5496
RRIDAB_3083973
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15355
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PPM1G antibody CL488-15532Download protocol
Standard Protocols
Click here to view our Standard Protocols