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PDP1 Recombinant antibody

PDP1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242090E7

Cat no : 84612-3-RR

Print datasheet

Synonyms

PPM2C, PDPC 1, PDP 1, EC:3.1.3.43, 242090E7

验证数据展示

  • WB analysis using 84612-3-RR

    WB analysis using 84612-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84612-3-RR

    Affinity and Kinetic Characterization of 84612-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inMDA-MB-231 cells, PC-3 cells, U-87 MG cells, mouse skeletal muscle tissue, mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy PDP1 Ready-To-Use IHC Kit. PDP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84612-3-RR targets PDP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PDP1 fusion protein Ag15453 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein phosphatase 2C, magnesium-dependent, catalytic subunit
Synonyms PPM2C, PDPC 1, PDP 1, EC:3.1.3.43, 242090E7
Calculated Molecular Weight 537 aa, 61 kDa
Observed Molecular Weight 55-61 kDa
GenBank Accession NumberBC047619
Gene Symbol PDP1
Gene ID (NCBI) 54704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P0J1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyruvate dehydrogenase phosphatase 1 (PDP1),also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

实验方案

Product Specific Protocols
WB protocol for PDP1 antibody 84612-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols