验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, PC-3 cells, U-87 MG cells, mouse skeletal muscle tissue, mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy PDP1 Ready-To-Use IHC Kit. PDP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84612-3-RR targets PDP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PDP1 fusion protein Ag15453 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2C, magnesium-dependent, catalytic subunit |
| 别名 | PPM2C, PDPC 1, PDP 1, EC:3.1.3.43, 242090E7 |
| 计算分子量 | 537 aa, 61 kDa |
| 观测分子量 | 55-61 kDa |
| GenBank蛋白编号 | BC047619 |
| 基因名称 | PDP1 |
| Gene ID (NCBI) | 54704 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9P0J1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pyruvate dehydrogenase phosphatase 1 (PDP1),also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDP1 antibody 84612-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |