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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83872-1-RR (same clone as 83872-1-PBS)

    WB analysis using 83872-1-RR (same clone as 83872-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83872-1-RR (PRDM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brown adipose using 83872-1-RR (same clone as 83872-1-PBS)

    IHC staining of mouse brown adipose using 83872-1-RR (same clone as 83872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83872-1-RR (PRDM16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDM16 antibody included
  • IF Staining of HEK-293 using 83872-1-RR (same clone as 83872-1-PBS)

    IF Staining of HEK-293 using 83872-1-RR (same clone as 83872-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PRDM16 antibody (83872-1-RR, Clone: 240662D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

PRDM16 Recombinant antibody, PBS Only

PRDM16 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83872-1-PBS

产品说明书

CloneNo. 240662D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRDM16 Ready-To-Use IHC Kit. PRDM16 primary antibody included.

产品信息

83872-1-PBS targets PRDM16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PR domain containing 16
别名 MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367
计算分子量 140 kDa
观测分子量~140 kDa
GenBank蛋白编号NM_022114
基因名称 PRDM16
Gene ID (NCBI) 63976
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HAZ2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.