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PRDM16 Recombinant antibody

PRDM16 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240662D3

Cat no : 83872-1-RR

Print datasheet

Synonyms

MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367

验证数据展示

  • WB analysis using 83872-1-RR

    WB analysis using 83872-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83872-1-RR (PRDM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 83872-1-RR

    IF Staining of HEK-293 using 83872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PRDM16 antibody (83872-1-RR, Clone: 240662D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inU2OS cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PRDM16 Ready-To-Use IHC Kit. PRDM16 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83872-1-RR targets PRDM16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PR domain containing 16
Synonyms MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367
Calculated Molecular Weight 140 kDa
Observed Molecular Weight~140 kDa
GenBank Accession NumberNM_022114
Gene Symbol PRDM16
Gene ID (NCBI) 63976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HAZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.

实验方案

Product Specific Protocols
WB protocol for PRDM16 antibody 83872-1-RRDownload protocol
IF protocol for PRDM16 antibody 83872-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols