CoraLite® Plus 488-conjugated PRDM16 Recombinant antibody
PRDM16 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240662D3
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy PRDM16 Ready-To-Use IHC Kit. PRDM16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83872-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83872 targets PRDM16 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | PR domain containing 16 |
| Synonyms | |
| Calculated Molecular Weight | 140 kDa |
| Observed Molecular Weight | ~140 kDa |
| GenBank Accession Number | NM_022114 |
| Gene Symbol | PRDM16 |
| Gene ID (NCBI) | 63976 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HAZ2 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRDM16 antibody CL488-83872 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |