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验证数据展示

  • WB analysis using 68365-1-Ig

    WB analysis using 68365-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 68365-1-Ig

    FC experiment of HeLa using 68365-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of A431 using 68365-1-Ig

    IF Staining of A431 using 68365-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (68365-1-Ig, Clone: 1G8E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

PRMT1 Monoclonal antibody

PRMT1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68365-1-Ig

Print datasheet

CloneNo. 1G8E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68365-1-Ig targets PRMT1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRMT1 fusion protein Ag29063 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein arginine methyltransferase 1
Synonyms HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight38~42 kDa
GenBank Accession NumberBC019268
Gene Symbol PRMT1
Gene ID (NCBI) 3276
RRIDAB_3085086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99873
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

实验方案

Product Specific Protocols
WB protocol for PRMT1 antibody 68365-1-IgDownload protocol
IF protocol for PRMT1 antibody 68365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols