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验证数据展示

  • WB analysis using 68365-1-Ig

    WB analysis using 68365-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 68365-1-Ig

    FC experiment of HeLa using 68365-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of A431 using 68365-1-Ig

    IF Staining of A431 using 68365-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (68365-1-Ig, Clone: 1G8E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

PRMT1 Monoclonal antibody

PRMT1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68365-1-Ig

产品说明书

CloneNo. 1G8E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68365-1-Ig targets PRMT1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRMT1 fusion protein Ag29063 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein arginine methyltransferase 1
别名 HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1
计算分子量 42 kDa
观测分子量38~42 kDa
GenBank蛋白编号BC019268
基因名称 PRMT1
Gene ID (NCBI) 3276
RRIDAB_3085086
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

实验方案

Product Specific Protocols
WB protocol for PRMT1 antibody 68365-1-IgDownload protocol
IF protocol for PRMT1 antibody 68365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols