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PRMT1 Monoclonal antibody, PBS Only

PRMT1 Monoclonal Antibody for WB, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8E2

Cat No : 68365-1-PBS

Print datasheet

Synonyms

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1

验证数据展示

  • WB analysis using 68365-1-Ig (same clone as 68365-1-PBS)

    WB analysis using 68365-1-Ig (same clone as 68365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68365-1-Ig (same clone as 68365-1-PBS)

    FC experiment of HeLa using 68365-1-Ig (same clone as 68365-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

产品信息

68365-1-PBS targets PRMT1 in WB, FC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, FC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen PRMT1 fusion protein Ag29063 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein arginine methyltransferase 1
Synonyms ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight38~42 kDa
GenBank Accession NumberBC019268
Gene Symbol PRMT1
Gene ID (NCBI) 3276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99873
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.