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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68365-1-Ig (same clone as 68365-1-PBS)

    WB analysis using 68365-1-Ig (same clone as 68365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68365-1-Ig (same clone as 68365-1-PBS)

    FC experiment of HeLa using 68365-1-Ig (same clone as 68365-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68365-1-Ig (same clone as 68365-1-PBS)

    IF Staining of A431 using 68365-1-Ig (same clone as 68365-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (68365-1-Ig, Clone: 1G8E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

PRMT1 Monoclonal antibody, PBS Only

PRMT1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68365-1-PBS

产品说明书

CloneNo. 1G8E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

产品信息

68365-1-PBS targets PRMT1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRMT1 fusion protein Ag29063 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein arginine methyltransferase 1
别名 HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1
计算分子量 42 kDa
观测分子量38~42 kDa
GenBank蛋白编号BC019268
基因名称 PRMT1
Gene ID (NCBI) 3276
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99873
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.