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CoraLite® Plus 488-conjugated PROS1 Polyclonal antibody

PROS1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16910

Print datasheet

Synonyms

PROS, PROS1, protein S (alpha), PS21, PS22, PS23, PS24, PS25, PSA, Vitamin K dependent protein S

验证数据展示

  • FC experiment of HepG2 using CL488-16910

    FC experiment of HepG2 using CL488-16910

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PROS1 (CL488-16910) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16910-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16910 targets PROS1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PROS1 fusion protein Ag10539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein S (alpha)
Synonyms PROS, PROS1, protein S (alpha), PS21, PS22, PS23, PS24, PS25, PSA, Vitamin K dependent protein S
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015801
Gene Symbol PROS1
Gene ID (NCBI) 5627
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07225
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PROS1 antibody CL488-16910Download protocol
Standard Protocols
Click here to view our Standard Protocols