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PSMA7 Monoclonal antibody

PSMA7 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C6

Cat No : 67817-1-Ig

Print datasheet

Synonyms

C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7

验证数据展示

  • WB analysis using 67817-1-Ig

    WB analysis using 67817-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human cervical cancer using 67817-1-Ig

    IHC staining of human cervical cancer using 67817-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA7 antibody included
  • IF Staining of HeLa using 67817-1-Ig

    IF Staining of HeLa using 67817-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of MCF-7 using 67817-1-Ig

    WB analysis of MCF-7 using 67817-1-Ig

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA7 Ready-To-Use IHC Kit. PSMA7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67817-1-Ig targets PSMA7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PSMA7 fusion protein Ag7821 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Synonyms C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC004427
Gene Symbol PSMA7
Gene ID (NCBI) 5688
RRIDAB_2918580
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

实验方案

Product Specific Protocols
WB protocol for PSMA7 antibody 67817-1-IgDownload protocol
IHC protocol for PSMA7 antibody 67817-1-IgDownload protocol
IF protocol for PSMA7 antibody 67817-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols