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  • Featured Product
  • KD/KO Validated

PSMA7 Monoclonal antibody, PBS Only

PSMA7 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1F2C6

Cat No : 67817-1-PBS

Print datasheet

Synonyms

C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7

验证数据展示

  • WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

    WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA7 antibody included
  • IF Staining of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    IF Staining of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMA7 Ready-To-Use IHC Kit. PSMA7 primary antibody included.

产品信息

67817-1-PBS targets PSMA7 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen PSMA7 fusion protein Ag7821 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Synonyms C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC004427
Gene Symbol PSMA7
Gene ID (NCBI) 5688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).