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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67847-1-Ig (same clone as 67847-1-PBS)

    WB analysis using 67847-1-Ig (same clone as 67847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67847-1-Ig (PSMB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67847-1-Ig (same clone as 67847-1-PBS)

    IHC staining of human liver cancer using 67847-1-Ig (same clone as 67847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67847-1-Ig (PSMB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB1 antibody included
  • IF Staining of U2OS using 67847-1-Ig (same clone as 67847-1-PBS)

    IF Staining of U2OS using 67847-1-Ig (same clone as 67847-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMB1 antibody (67847-1-Ig, Clone: 1C7C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67847-1-Ig (same clone as 67847-1-PBS)

    FC experiment of Jurkat using 67847-1-Ig (same clone as 67847-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PSMB1 Monoclonal antibody (67847-1-Ig, Clone:1C7C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

PSMB1 Monoclonal antibody, PBS Only

PSMB1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67847-1-PBS

产品说明书

CloneNo. 1C7C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

1C7C5, FLJ25321, HC5, KIAA1838, Macropain subunit C5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.

产品信息

67847-1-PBS targets PSMB1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PSMB1 fusion protein Ag30791 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 1
别名 1C7C5, FLJ25321, HC5, KIAA1838, Macropain subunit C5
计算分子量 241 aa, 26 kDa
观测分子量25 kDa
GenBank蛋白编号BC020807
基因名称 PSMB1
Gene ID (NCBI) 5689
RRIDAB_2918609
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20618
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.