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PSMB1 Monoclonal antibody, PBS Only

PSMB1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7C5

Cat no : 67847-1-PBS

Print datasheet

Synonyms

FLJ25321, HC5, KIAA1838, Macropain subunit C5, PMSB1, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta type 1, PSC5, PSMB1

验证数据展示

  • WB analysis using 67847-1-Ig (same clone as 67847-1-PBS)

    WB analysis using 67847-1-Ig (same clone as 67847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67847-1-Ig (PSMB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67847-1-Ig (same clone as 67847-1-PBS)

    IHC staining of human liver cancer using 67847-1-Ig (same clone as 67847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67847-1-Ig (PSMB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB1 antibody included
  • IF Staining of U2OS using 67847-1-Ig (same clone as 67847-1-PBS)

    IF Staining of U2OS using 67847-1-Ig (same clone as 67847-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMB1 antibody (67847-1-Ig, Clone: 1C7C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67847-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.

产品信息

67847-1-PBS targets PSMB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen PSMB1 fusion protein Ag30791 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 1
Synonyms FLJ25321, HC5, KIAA1838, Macropain subunit C5, PMSB1, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta type 1, PSC5, PSMB1
Calculated Molecular Weight 241 aa, 26 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC020807
Gene Symbol PSMB1
Gene ID (NCBI) 5689
RRIDAB_2918609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20618
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.