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验证数据展示

  • WB analysis using 67847-1-Ig

    WB analysis using 67847-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67847-1-Ig (PSMB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 67847-1-Ig

    IHC staining of human liver cancer using 67847-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67847-1-Ig (PSMB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB1 antibody included
  • IF Staining of U2OS using 67847-1-Ig

    IF Staining of U2OS using 67847-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMB1 antibody (67847-1-Ig, Clone: 1C7C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 67847-1-Ig

    FC experiment of Jurkat using 67847-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PSMB1 Monoclonal antibody (67847-1-Ig, Clone:1C7C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PSMB1 Monoclonal antibody

PSMB1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67847-1-Ig

产品说明书

CloneNo. 1C7C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

1C7C5, FLJ25321, HC5, KIAA1838, Macropain subunit C5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67847-1-Ig targets PSMB1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PSMB1 fusion protein Ag30791 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 1
别名 1C7C5, FLJ25321, HC5, KIAA1838, Macropain subunit C5
计算分子量 241 aa, 26 kDa
观测分子量25 kDa
GenBank蛋白编号BC020807
基因名称 PSMB1
Gene ID (NCBI) 5689
RRIDAB_2918609
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for PSMB1 antibody 67847-1-IgDownload protocol
IHC protocol for PSMB1 antibody 67847-1-IgDownload protocol
IF protocol for PSMB1 antibody 67847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols