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验证数据展示

  • FC experiment of HeLa using CL488-67847

    FC experiment of HeLa using CL488-67847

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PSMB1 (CL488-67847, Clone:1C7C5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67847

    IF Staining of HeLa using CL488-67847

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PSMB1 antibody (CL488-67847, Clone: 1C7C5 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PSMB1 Monoclonal antibody

PSMB1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67847

产品说明书

CloneNo. 1C7C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PMSB1, Macropain subunit C5, KIAA1838, HC5, FLJ25321

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67847-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67847 targets PSMB1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PSMB1 fusion protein Ag30791 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 1
别名 PMSB1, Macropain subunit C5, KIAA1838, HC5, FLJ25321
计算分子量 241 aa, 26 kDa
观测分子量25 kDa
GenBank蛋白编号BC020807
基因名称 PSMB1
Gene ID (NCBI) 5689
RRIDAB_2923802
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20618
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSMB1 antibody CL488-67847Download protocol
FC protocol for CL Plus 488 PSMB1 antibody CL488-67847Download protocol
Standard Protocols
Click here to view our Standard Protocols