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CoraLite®594-conjugated PSMB1 Monoclonal antibody

PSMB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C7C5

Cat no : CL594-67847

Print datasheet

Synonyms

PMSB1, Macropain subunit C5, KIAA1838, HC5, FLJ25321

验证数据展示

  • FC experiment of HeLa using CL594-67847

    FC experiment of HeLa using CL594-67847

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMB1 (CL594-67847, Clone:1C7C5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67847-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67847 targets PSMB1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PSMB1 fusion protein Ag30791 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 1
Synonyms PMSB1, Macropain subunit C5, KIAA1838, HC5, FLJ25321
Calculated Molecular Weight 241 aa, 26 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC020807
Gene Symbol PSMB1
Gene ID (NCBI) 5689
RRIDAB_3084788
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20618
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

实验方案

Product Specific Protocols
FC protocol for CL594 PSMB1 antibody CL594-67847Download protocol
Standard Protocols
Click here to view our Standard Protocols