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PSMD3 Recombinant antibody

PSMD3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242470D4

Cat no : 84928-2-RR

Print datasheet

Synonyms

TSTA2, S3, RPN3, P58, 26S proteasome non-ATPase regulatory subunit 3

验证数据展示

  • WB analysis using 84928-2-RR

    WB analysis using 84928-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 84928-2-RR

    FC experiment of A549 using 84928-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, U2OS cells, U-251 cells
Positive FC (Intra) detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD3 Ready-To-Use IHC Kit. PSMD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84928-2-RR targets PSMD3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PSMD3 fusion protein Ag2684 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Synonyms TSTA2, S3, RPN3, P58, 26S proteasome non-ATPase regulatory subunit 3
Calculated Molecular Weight 534 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC012302
Gene Symbol PSMD3
Gene ID (NCBI) 5709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43242
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

实验方案

Product Specific Protocols
WB protocol for PSMD3 antibody 84928-2-RRDownload protocol
FC protocol for PSMD3 antibody 84928-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols