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验证数据展示

  • WB analysis using 84657-4-RR

    WB analysis using 84657-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84657-4-RR (PSPC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84657-4-RR

    Affinity and Kinetic Characterization of 84657-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84657-4-RR against Human PSPC1 were performed. The affinity constant is 0.68 nM.

PSPC1 Recombinant antibody

PSPC1 Recombinant Antibody for WB, ELISA
Cat No. 84657-4-RR

产品说明书

CloneNo. 242158G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

PSPC 1, PSP1, 242158G7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, PC-3 cells, MDA-MB-453 cells
Planning an IHC experiment? We recommend our IHCeasy PSPC1 Ready-To-Use IHC Kit. PSPC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84657-4-RR targets PSPC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PSPC1 fusion protein Ag10136 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paraspeckle component 1
别名 PSPC 1, PSP1, 242158G7
计算分子量 523 aa, 59 kDa
观测分子量 66 kDa
GenBank蛋白编号BC014184
基因名称 PSPC1
Gene ID (NCBI) 55269
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8WXF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

实验方案

Product Specific Protocols
WB protocol for PSPC1 antibody 84657-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols