验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, DU 145 cells, HT-29 cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
60300-3-Ig targets PTEN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | PTEN fusion protein Ag17274 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphatase and tensin homolog |
| Synonyms | EC:3.1.3.-, BZS, BC005821, Bannayan Zonana syndrome, 4D8G9 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC005821 |
| Gene Symbol | PTEN |
| Gene ID (NCBI) | 5728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60484 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTEN antibody 60300-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunother Taurine Inhibits Lung Metastasis in Triple-Negative Breast Cancer by Modulating Macrophage Polarization Through PTEN-PI3K/Akt/mTOR Pathway | ||
Cell Death Discov Myeloid PTEN loss affects the therapeutic response by promoting stress granule assembly and impairing phagocytosis by macrophages in breast cancer |