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验证数据展示

  • WB analysis using 60300-3-Ig (same clone as 60300-3-PBS)

    WB analysis using 60300-3-Ig (same clone as 60300-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60300-3-Ig (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60300-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50170-1

    Cytometric bead array standard curve of MP50170-1

    Cytometric bead array standard curve of MP50170-1, PTEN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60300-2-PBS. Detection antibody: 60300-3-PBS. Standard:Ag17274. Range: 0.391-12.5 ng/mL.

PTEN Monoclonal antibody, PBS Only (Detector)

PTEN Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 60300-3-PBS

产品说明书

CloneNo. 4D8G9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

10q23del, 4D8G9, Bannayan Zonana syndrome, BC005821, BZS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.

产品信息

60300-3-PBS targets PTEN as part of a matched antibody pair:

MP50170-1: 60300-2-PBS capture and 60300-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PTEN fusion protein Ag17274 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatase and tensin homolog
别名 10q23del, 4D8G9, Bannayan Zonana syndrome, BC005821, BZS
计算分子量 47 kDa
观测分子量 55 kDa
GenBank蛋白编号BC005821
基因名称 PTEN
Gene ID (NCBI) 5728
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60484
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.